Use of an in vitro fermentation bioassay to evaluate improvements in degradation of alfalfa hay due to exogenous feed enzymes

An in vitro batch culture assay was used to determine the efficacy of five developmental feed enzyme products in terms of improving ruminal degradation of alfalfa hay. A second objective was to establish whether the length of time that alfalfa hay was incubated with ruminal fluid and buffer in a batch culture in vitro system influenced enzyme efficacy. The experiment was conducted as a completely randomized design in four replications with treatments arranged as a 3 (incubation times)z5 (enzyme products) factorial. Two of the enzyme products (P1 and P2) were proteases, while the other three products (F1, F2, and F3) contained differing proportions of endoglucanases and xylanases. Milled alfalfa hay was incubated with buffer, ruminal fluid and enzyme product (1.5 mg/g of dry matter, DM). Gas production (GP) and degradability of DM and fibre were measured after terminating the incubation at 12, 18 and 24 h. At all incubation times, P1 increased GP by 5.6-7.9%, but GP was not affected by P2. Of the polysaccharidases evaluated, F1 and F2 increased GP by 3.7-10.6% at all incubation times, while F3 had no effect. Improvements in fibre degradability depended upon enzyme product and incubation time. Degradation of neutral detergent fibre (aNDF) was increased at 12 h of incubation using F1 and F2 (12-13% increase), at 18 h of incubation using P1 (11%) and F1 (7%), and at 24 h by F1 (10.5%), F2 (16.5%), and F3 (11.3%). Improvements in acid detergent fibre degradation ranged from 17.5 to 44.4% for these enzymes, depending upon incubation time

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Product / service:	Carbohydrases and Proteases
Publication date:	01/01/2007
Species:	Ruminants
Authors:	Eun JS, Beauchemin KA, Schulze H
Doctype:	Publications & Citations
Publication / conference:	Animal Feed Science & Technology, volume 135
Regions and countries:	Global
Keywords:	alfalfa, hay, batch, culture, buffer, degradability, degradation, detergent, dm, dry, matter, efficacy, endoglucanase, endoglucanases, enzyme, enzymes, feed, fermentation, fibre, gas, production, in, vitro, in-vitro, incubation, length, neutral, products, protease, proteases, replication, ruminal, fluid, system, time, treatment, xylanase, xylanases
Production challenge(s):	Gut health
Diets:	Silage
Brands:	Axtra® XAP, Danisco Xylanase
Resource ref:	10087
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Use of an in vitro fermentation bioassay to evaluate improvements in degradation of alfalfa hay due to exogenous feed enzymes

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