The efficacy of an Escherichia coli-derived phytase preparation

Five experiments were conducted to evaluate the effect of an Escherichia coli-derived phytase on phytate-P use and growth performance by young pigs. The first experiment involved time course, pH dependence, and phytase activity studies to investigate the in vitro release of P from corn, soybean meal, and an inorganic P-unsupplemented corn-soybean meal negative control diet. In Exp. 2, which was designed to determine the efficacy of the E. coli-derived vs. fungal phytase-added diets at 0, 250, 500, 750, 1,000, or 1,250FTU/kg (as-fed basis, one phytase unit or FTU is de-fined as the quantity of enzyme required to liberate 1mol of inorganic P/min, at pH 5.5, from an excess of 15 M sodium phytate at 37oC) and a positive control diet, eight individually penned 10-kg pigs per diet (12 diets, 96 pigs) were used in a 28-d growth study. The third experiment was a 10-d nutrient balance study involving six 13-kg pigs per diet (four diets, 24 pigs) in individual metabolism crates. In Exp. 4, eight pens (four pigs per pen) of 19-kg pigs per treatment were used in a 42-d growth performance study to examine the effect of adding the E. coli-derived phytase to corn soybean diets at 0, 500, or 1,000 FTU/kg (as-fed basis) and a positive control (four diets, 128 pigs). In Exp. 5, six 19-kg pigs per treatment were used in a 10-d nutrient balance study to investigate the effects of the E. coli derived phytase added to diets at 0, 250, 500, 750, or 1,000 FTU/kg (as-fed basis) and a positive control diet (six diets, 36 pigs). The in vitro study showed that the E. coli-derived phytase has an optimal activity and pH range of 2 to 4.5. Inorganic phosphate release was greatest for soybean meal, least for corn, and intermediate for the negative control diet. Dietary supplementation with graded amounts of E. coli-derived phytase resulted in linear increases (P < 0.05) in weight gain, feed efficiency, and plasma Ca and P concentrations in 10-kg pigs in Exp. 2. Phytase also increased P digestibility and retention in the 13-kg pigs in Exp. 3. In Exp. 4, dietary supplementation with E. coli-derived phytase resulted in linear increases (P < 0.05) in weight gain and feed efficiency of 19-kg pigs. Supplementation of the diets of 19-kg pigs with the E. coli-derived phytase also improved Ca and P digestibility and retention in Exp. 5. In the current study, the new E. coli-derived phytase was efficacious in hydrolyzing phytate-P, both in vitro and in vivo, in young pigs

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Product / service:	Phytases
Publication date:	01/01/2004
Species:
Authors:	Adeola O, Sands JS, Simmins PH, Schulze H
Doctype:	Publications & Citations
Publication / conference:	Journal of Animal Science, volume 82
Regions and countries:	Global
Keywords:	activity, balance, studies, ca, concentration, control, corn, dan, de, diet, dietary, diets, digestibilities, digestibility, e, e.coli, phytase, effects, efficacy, efficiency, enzyme, feed, fungal, gain, growth, performance, in, vitro, vivo, in-vitro, key, meal, metabolism, nutrient, p, p-digestibility, per, ph, phosphate, phosphorus, phytate, pig, pigs, plasma, preparation, quantity, retention, sodium, soybean, supplementation, time, treatment, weight, young
Production challenge(s):	Gut health
Diets:	All diets
Brands:	Axtra® PHY, Phyzyme® XP, Optimize Feed, Phycheck, FASTKit assay
Resource ref:	8093
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The efficacy of an Escherichia coli-derived phytase preparation

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