Acid expression of carbohydrase and transporter in chickens Effect of diet containing phytate and phytase on the activity and messenger ribonucleic

    The effect of dietary phytate and phytase on carbohydrase activity and hexose transport was investigated in broiler chickens. Diets containing phytate P (2.2 or 4.4 g/kg) with different phytase dose rates (0, 500, or 1,000 phytase units/kg) were fed to 504 female Cobb chicks for 3 wk. Diets containing high phytate concentrations depressed (P < 0.05) BW and G:F, whereas phytase supplementation improved (P< 0.05) the performance of birds. In the duodenum, phytate decreased (P < 0.05) the activities of disaccharidases, Na+K+-ATPase, and glucose concentrations by 5 to 11%, but phytase enhanced (P < 0.05) the concentrations of amylase, sucrase, maltase, Na+K+-ATPase, and glucose by 5 to 30%. In the jejunum, phytate decreased (P < 0.05) the concentrations of amylase, sucrase, Na+K+-ATPase, and glucose by 10 to 22%, and phytase alleviated the negative effect of phytate on the above variables. Ingestion of diets containing phytate also decreased (P < 0.05) serum amylase activity and glucose concentration, and phytase enhanced maltase, Na+K+-ATPase, and glucose. There were also interactions (P < 0.05) between phytate and phytase on the concentrations of serum amylase, duodenal amylase, sucrase, and jejunal glucose. Enzymatic analysis at a molecular level showed that neither phytate nor phytase influenced the mRNA expression of sucraseisomaltase in the small intestine. Also, the investigation into the sodium glucose co transporter gene may challenge the mechanism by which phytate interferes with glucose utilization, as partly indicated by bird performance, and transmembrane transport because diets containing increased phytate up regulated (P < 0.05) the mRNA expression of the sodium glucose co transporter gene in duodenum and did not influence it in the jejunum. These results indicate that phytate can impair endogenous carbohydrase activity and digestive competence, and phytase can ameliorate these effects for chickens
    Document information
    Product / service: Phytases
    Publication date: 01/01/2008
    Species: Poultry, Broiler
    Authors: Liu N, Ru YJ, Li FD, Cowieson AJ
    Doctype: Publications & Citations
    Publication / conference: Journal of Animal Science, volume 86
    Regions and countries: Global
    Keywords: activity, amylase, analysis, birds, broiler, chicken, chickens, bw, carbohydrase, challenge, chick, chicks, concentration, diet, dietary, diets, digestive, disaccharidase, disaccharidases, dose, rate, duodenum, effects, endogenous, enzymatic, expression, female, gene, glucose, interaction, interactions, intestine, jejunum, maltase, mechanism, mrna, p, performance, phytase, phytate, serum, concentrations, small, sodium, sucrase, supplementation, transport, utilization
    Production challenge(s): Gut health
    Diets: All diets
    Brands: Axtra® PHY, Phyzyme® XP, Optimize Feed, Phycheck, FASTKit assay
    Resource ref: 10097
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