A simple and fast kinetic assay for phytases using phytic acid–protein complex as substrate

The most commonly used method to assay phytase, developed nearly a century ago, measures the Pi liberated from IP6. This traditional endpoint assay is time-consuming and well known for its cumbersomeness in addition to requiring extra caution for handling the toxic regents used. This article reports a simple, fast, and nontoxic kinetic method adaptable for high throughput for assaying phytase using IP6–lysozyme as a substrate.

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Product / service:	Enzymes, Phytases
Publication date:	02/11/2010
Species:	Poultry, Pig, Ruminants, Aquaculture
Authors:	Dalsgaard, S, Yu S, Hatti-Kaul, R, Tran, TT
Doctype:	Publications & Citations
Publication / conference:	Analytical Biochemistry
Regions and countries:	Global
Keywords:	endogenous enzyme, phosphorous tax, broiler multi-enzyme, broiler bodyweight gain, Kinetic assay for phytases, Microbial phytases, phytate poultry diets, piglet bodyweight gain, pig enzymes, poultry amylase, poultry b-glucanase, poultry carcass yield, poultry endogenous enzymes, poultry gut health, poultry phosphorus, Poultry phytase, poultry probiotics, poultry protease, poultry wheat diet, poultry xylanase, Rajni Hatti-Kaul, Shukun Yu, swine phytase, swine amylase, swine betaine, swine enzymes, swine FCR, swine profitability, swine protease, swine xylanase, Søren Dalsgaard, Thuy Thi Tran, Phytate–protein interaction
Production challenge(s):	animal producer profitability, Performance growth
Diets:	All diets
Brands:	Axtra® PHY, FASTKit assay, Optimize Feed, Phyzyme® XP
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